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DNA sequencing: Difference between revisions

411 bytes added ,  2 years ago
Added more sequencing information
(Added more sequencing information)
[https://en.wikipedia.org/wiki/Sanger_sequencing Sanger sequencing]
If the gel electrophoresis indicates that the PCR reaction worked, it can be brought in for sequencing. For sequencing I use [http://www.genewiz.com/ Genewiz], which haswill apick sampleup dropsamples offdirectly boxfrom CCL. If you fill out the sequencing order by 3:30pm, the courier arrives around five and you will get the sequences at 626about 6am Bancroftthe innext Berkeleymorning. If you sign up for a new account, the first two sequences are free. If you have Genewiz do the DNA quantification and PCR cleanup, they charge $7 per sample or $5 per sample if you do 96 at a time.
[http://www.genewiz.com/public/Sample-Submission-Guideline.aspx Genewiz sample submission guidelines]
[http://www.mclab.com/DNA-Sequencing-Services.html MClab does DNA sequencing less expensively]
Lower cost sequencing options are available, though you need to mail in the PCR products rather than having them picked up. Two low cost Sanger sequencing options are [https://www.eurofinsgenomics.com/en/products/dna-sequencing/all-sequencing-options/ Eurofins] and [http://www.mclab.com/DNA-Sequencing-Services.html McLab].
[http://www.mclab.com/DNA-Sequencing-Services.html MClab does DNA sequencing less expensively]
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