DNA sequencing: Difference between revisions

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== Overview ==
 
DNA barcoding enables the identification of unknown organisms, discovery of new species, the determination of which features delineate species, accurate determination of the range of a species and the building of phylogenetic trees which show how various organisms are related. The most powerful thing that DNA barcoding does is it tells you which collections are the same species. If your goal is to get insights into strains of the same species or learn which genes are present, you'll need to use a different process called full genome sequencing.
 
Step by step photos of the whole process are available here: https://www.facebook.com/groups/FungalSequencing/permalink/2268745516680176/
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Back to basics: an evaluation of NaOH and alternative rapid DNA extraction protocols for DNA barcoding, genotyping, and disease diagnostics from fungal and oomycete samples https://nature.berkeley.edu/garbelotto/downloads/naohextraction2013.pdf
 
In a pinch, plain water can be used as DNA extraction solution.
 
== Polymerase Chain Reaction (PCR) ==