DNA sequencing: Difference between revisions
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The goal of DNA extraction is to get a few nanograms of your organism's DNA into solution so it can be used as a [https://en.wikipedia.org/wiki/Polymerase_chain_reaction PCR template].
Two good DNA extraction methods are the NaOH extraction and [http://www.sigmaaldrich.com/life-science/molecular-biology/dna-and-rna-purification/extract-n-amp.html ExtractNAmp]. In a pinch you can just use water.
* NaOH extraction (Wang et al. 1993, Osmundson et al. 2013). 200 μL of 0.5 M NaOH is added to the ground tissue. 5 μL of the extract is diluted in 495 μL of 100 mM Tris-HCl, pH 8.0 (pH is adjusted with pH meter and HCl until it is 8.0), and 1 μL of the dilution is used as template DNA for a 25 μL PCR reaction.
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