DNA sequencing: Difference between revisions
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(→DNA Extraction: Added good links to DNA extraction papers) |
(Added primer maps and sequences) |
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[http://tmcalculator.neb.com Annealing temperature calculator] |
[http://tmcalculator.neb.com Annealing temperature calculator] |
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[https://www2.clarku.edu/faculty/dhibbett/Protocols_Folder/Primers/Primers.pdf Primer maps and sequences] ( for loci ITS, LSU, SSU, TEF1, RPB1, RPB2, COX3, ATP6 ) |
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I use a 25 μL [https://en.wikipedia.org/wiki/Polymerase_chain_reaction PCR] reaction which includes 1 μL of 10 μM forward [https://en.wikipedia.org/wiki/Primer_(molecular_biology) primer], 1 μL of 10 μM reverse primer, 1 μL DNA template and the [https://www.google.com/search?q=pcr+master+mix&oq=PCR+master+mix PCR master mix]. I use the [https://www.the-odin.com/taq-polymerase-master-mix-2x-1ml-40-reactions/ TAQ master mix available at The Odin]. |
I use a 25 μL [https://en.wikipedia.org/wiki/Polymerase_chain_reaction PCR] reaction which includes 1 μL of 10 μM forward [https://en.wikipedia.org/wiki/Primer_(molecular_biology) primer], 1 μL of 10 μM reverse primer, 1 μL DNA template and the [https://www.google.com/search?q=pcr+master+mix&oq=PCR+master+mix PCR master mix]. I use the [https://www.the-odin.com/taq-polymerase-master-mix-2x-1ml-40-reactions/ TAQ master mix available at The Odin]. |
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