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Revision as of 08:13, 10 December 2022 (edit) Alan Rockefeller (talk \| contribs) No edit summary Tag: Visual edit ← Older edit		Revision as of 18:42, 3 January 2023 (edit) (undo) Alan Rockefeller (talk \| contribs) (More information on Sanger sequencing) Tag: Visual edit Newer edit →
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	Lower cost sequencing options are available, though you need to mail in the PCR products rather than having them picked up. Two low cost Sanger sequencing options are [https://www.eurofinsgenomics.com/en/products/dna-sequencing/all-sequencing-options/ Eurofins] and [http://www.mclab.com/DNA-Sequencing-Services.html McLab].		Lower cost sequencing options are available, though you need to mail in the PCR products rather than having them picked up. Two low cost Sanger sequencing options are [https://www.eurofinsgenomics.com/en/products/dna-sequencing/all-sequencing-options/ Eurofins] and [http://www.mclab.com/DNA-Sequencing-Services.html McLab]. I think the best way to do it is do slightly larger reactions - maybe 35 uL, and then use McLab for both forward and reverse reads. It costs $6 per sample, $4 less than just one read from Genewiz. Often the forward works and the reverse doesn't, or vice versa - usually one of them works unless you have multiple templates being amplified or some other problem. Slightly larger reactions because McLab requests larger sample sizes than Genewiz, and you need to submit forward and reverse reads separately.