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IGEM team meeting notes 5 26 14
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== Gibson == three enzymes used for every reaction (more costly) one-step process Like SLIC except it uses T5 exonuclease (5' --> 3' activity; opposite of T4 used in SLIC) nicks repaired before they go into e. coli overhangs must lack secondary structure to avoid formation of hairpins which would compete with GOI for insertion into backbone only method with a theme song :)
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