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IGEM team meeting notes 5 26 14
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== SLIC: sequence and ligation independent cloning == one enzyme used for every reaction multi-step process; backbone vector contains sequences intrinsic to the plasmid backbone; GOI designed with same DNA at both ends throw in T4 DNA polymerase because it has 3' DNA exonuclease activity; eats up DNA from opposite ends of template strands e.coli (for example) repair machinery fills in gaps after ligation make constructs in e.coli and then express them in yeast
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